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primary antibodies recognizing eea1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc primary antibodies recognizing eea1
    A. HeLa S3 cells were transfected with scrambled control (siScram) or BICD2-targeting (siBICD2) siRNAs and infected with HPV harboring the luciferase reporter plasmid at the MOI of ~200. At 16, 24, and 32 hpi, PLA was performed with antibodies recognizing HPV L1 and <t>EEA1.</t> Mock, uninfected; HPV, infected. PLA signals are green; nuclei are blue (DAPI). Similar results were obtained in two independent experiments. B. The fluorescence of PLA signals was determined from multiple images obtained as in (A). Each dot represents an individual cell (n>40) and black horizontal lines indicate the mean value of the analyzed population in each group. ***p < 0.001; ****p < 0.0001; ns, not significant. The graph shows results of a representative experiment. C. As in (A) except PLA was performed using antibodies recognizing HPV L1 and TGN46. D. As in (B) from multiple images obtained as in (C). **p < 0.01. E. Proposed model for dynein-BICD2 dependent transport of HPV16 during entry. The dynein-BICD2 complex first engages HPV L2 after cytosolic exposure of L2 from the endosome. This interaction helps to transport the virus from the endosome to the TGN/Golgi. The BICD2-dynein complex continues to ferry HPV through the Golgi and eventually to the nucleus for infection. Figure created in BioRender. Blue, BICD2; orange, dynein; red, L2; green, microtubule; light blue, membrane lipid bilayer. BICD2 siRNA #2 was used.
    Primary Antibodies Recognizing Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 483 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies recognizing eea1/product/Cell Signaling Technology Inc
    Average 96 stars, based on 483 article reviews
    primary antibodies recognizing eea1 - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "The BICD2 dynein cargo adaptor binds to the HPV16 L2 capsid protein and promotes HPV infection"

    Article Title: The BICD2 dynein cargo adaptor binds to the HPV16 L2 capsid protein and promotes HPV infection

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1012289

    A. HeLa S3 cells were transfected with scrambled control (siScram) or BICD2-targeting (siBICD2) siRNAs and infected with HPV harboring the luciferase reporter plasmid at the MOI of ~200. At 16, 24, and 32 hpi, PLA was performed with antibodies recognizing HPV L1 and EEA1. Mock, uninfected; HPV, infected. PLA signals are green; nuclei are blue (DAPI). Similar results were obtained in two independent experiments. B. The fluorescence of PLA signals was determined from multiple images obtained as in (A). Each dot represents an individual cell (n>40) and black horizontal lines indicate the mean value of the analyzed population in each group. ***p < 0.001; ****p < 0.0001; ns, not significant. The graph shows results of a representative experiment. C. As in (A) except PLA was performed using antibodies recognizing HPV L1 and TGN46. D. As in (B) from multiple images obtained as in (C). **p < 0.01. E. Proposed model for dynein-BICD2 dependent transport of HPV16 during entry. The dynein-BICD2 complex first engages HPV L2 after cytosolic exposure of L2 from the endosome. This interaction helps to transport the virus from the endosome to the TGN/Golgi. The BICD2-dynein complex continues to ferry HPV through the Golgi and eventually to the nucleus for infection. Figure created in BioRender. Blue, BICD2; orange, dynein; red, L2; green, microtubule; light blue, membrane lipid bilayer. BICD2 siRNA #2 was used.
    Figure Legend Snippet: A. HeLa S3 cells were transfected with scrambled control (siScram) or BICD2-targeting (siBICD2) siRNAs and infected with HPV harboring the luciferase reporter plasmid at the MOI of ~200. At 16, 24, and 32 hpi, PLA was performed with antibodies recognizing HPV L1 and EEA1. Mock, uninfected; HPV, infected. PLA signals are green; nuclei are blue (DAPI). Similar results were obtained in two independent experiments. B. The fluorescence of PLA signals was determined from multiple images obtained as in (A). Each dot represents an individual cell (n>40) and black horizontal lines indicate the mean value of the analyzed population in each group. ***p < 0.001; ****p < 0.0001; ns, not significant. The graph shows results of a representative experiment. C. As in (A) except PLA was performed using antibodies recognizing HPV L1 and TGN46. D. As in (B) from multiple images obtained as in (C). **p < 0.01. E. Proposed model for dynein-BICD2 dependent transport of HPV16 during entry. The dynein-BICD2 complex first engages HPV L2 after cytosolic exposure of L2 from the endosome. This interaction helps to transport the virus from the endosome to the TGN/Golgi. The BICD2-dynein complex continues to ferry HPV through the Golgi and eventually to the nucleus for infection. Figure created in BioRender. Blue, BICD2; orange, dynein; red, L2; green, microtubule; light blue, membrane lipid bilayer. BICD2 siRNA #2 was used.

    Techniques Used: Transfection, Control, Infection, Luciferase, Plasmid Preparation, Fluorescence, Virus, Membrane

    Antibodies and inhibitors.
    Figure Legend Snippet: Antibodies and inhibitors.

    Techniques Used: Solvent



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    primary antibodies recognizing eea1  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc primary antibodies recognizing eea1
    A. HeLa S3 cells were transfected with scrambled control (siScram) or BICD2-targeting (siBICD2) siRNAs and infected with HPV harboring the luciferase reporter plasmid at the MOI of ~200. At 16, 24, and 32 hpi, PLA was performed with antibodies recognizing HPV L1 and <t>EEA1.</t> Mock, uninfected; HPV, infected. PLA signals are green; nuclei are blue (DAPI). Similar results were obtained in two independent experiments. B. The fluorescence of PLA signals was determined from multiple images obtained as in (A). Each dot represents an individual cell (n>40) and black horizontal lines indicate the mean value of the analyzed population in each group. ***p < 0.001; ****p < 0.0001; ns, not significant. The graph shows results of a representative experiment. C. As in (A) except PLA was performed using antibodies recognizing HPV L1 and TGN46. D. As in (B) from multiple images obtained as in (C). **p < 0.01. E. Proposed model for dynein-BICD2 dependent transport of HPV16 during entry. The dynein-BICD2 complex first engages HPV L2 after cytosolic exposure of L2 from the endosome. This interaction helps to transport the virus from the endosome to the TGN/Golgi. The BICD2-dynein complex continues to ferry HPV through the Golgi and eventually to the nucleus for infection. Figure created in BioRender. Blue, BICD2; orange, dynein; red, L2; green, microtubule; light blue, membrane lipid bilayer. BICD2 siRNA #2 was used.
    Primary Antibodies Recognizing Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies recognizing eea1/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    primary antibodies recognizing eea1 - by Bioz Stars, 2026-06
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    A. HeLa S3 cells were transfected with scrambled control (siScram) or BICD2-targeting (siBICD2) siRNAs and infected with HPV harboring the luciferase reporter plasmid at the MOI of ~200. At 16, 24, and 32 hpi, PLA was performed with antibodies recognizing HPV L1 and EEA1. Mock, uninfected; HPV, infected. PLA signals are green; nuclei are blue (DAPI). Similar results were obtained in two independent experiments. B. The fluorescence of PLA signals was determined from multiple images obtained as in (A). Each dot represents an individual cell (n>40) and black horizontal lines indicate the mean value of the analyzed population in each group. ***p < 0.001; ****p < 0.0001; ns, not significant. The graph shows results of a representative experiment. C. As in (A) except PLA was performed using antibodies recognizing HPV L1 and TGN46. D. As in (B) from multiple images obtained as in (C). **p < 0.01. E. Proposed model for dynein-BICD2 dependent transport of HPV16 during entry. The dynein-BICD2 complex first engages HPV L2 after cytosolic exposure of L2 from the endosome. This interaction helps to transport the virus from the endosome to the TGN/Golgi. The BICD2-dynein complex continues to ferry HPV through the Golgi and eventually to the nucleus for infection. Figure created in BioRender. Blue, BICD2; orange, dynein; red, L2; green, microtubule; light blue, membrane lipid bilayer. BICD2 siRNA #2 was used.

    Journal: PLOS Pathogens

    Article Title: The BICD2 dynein cargo adaptor binds to the HPV16 L2 capsid protein and promotes HPV infection

    doi: 10.1371/journal.ppat.1012289

    Figure Lengend Snippet: A. HeLa S3 cells were transfected with scrambled control (siScram) or BICD2-targeting (siBICD2) siRNAs and infected with HPV harboring the luciferase reporter plasmid at the MOI of ~200. At 16, 24, and 32 hpi, PLA was performed with antibodies recognizing HPV L1 and EEA1. Mock, uninfected; HPV, infected. PLA signals are green; nuclei are blue (DAPI). Similar results were obtained in two independent experiments. B. The fluorescence of PLA signals was determined from multiple images obtained as in (A). Each dot represents an individual cell (n>40) and black horizontal lines indicate the mean value of the analyzed population in each group. ***p < 0.001; ****p < 0.0001; ns, not significant. The graph shows results of a representative experiment. C. As in (A) except PLA was performed using antibodies recognizing HPV L1 and TGN46. D. As in (B) from multiple images obtained as in (C). **p < 0.01. E. Proposed model for dynein-BICD2 dependent transport of HPV16 during entry. The dynein-BICD2 complex first engages HPV L2 after cytosolic exposure of L2 from the endosome. This interaction helps to transport the virus from the endosome to the TGN/Golgi. The BICD2-dynein complex continues to ferry HPV through the Golgi and eventually to the nucleus for infection. Figure created in BioRender. Blue, BICD2; orange, dynein; red, L2; green, microtubule; light blue, membrane lipid bilayer. BICD2 siRNA #2 was used.

    Article Snippet: Primary antibodies recognizing EEA1 (Cell Signaling Technology, 2411) or TGN46 (Abcam, ab50595) were diluted with DMEM10 (1:250) and incubated with the coverslips overnight at 4°C.Secondary antibodies were diluted with TBS containing 0.2% Tween-20 and 3% BSA or DMEM10 and incubated with coverslips for one hour at room temperature.

    Techniques: Transfection, Control, Infection, Luciferase, Plasmid Preparation, Fluorescence, Virus, Membrane

    Antibodies and inhibitors.

    Journal: PLOS Pathogens

    Article Title: The BICD2 dynein cargo adaptor binds to the HPV16 L2 capsid protein and promotes HPV infection

    doi: 10.1371/journal.ppat.1012289

    Figure Lengend Snippet: Antibodies and inhibitors.

    Article Snippet: Primary antibodies recognizing EEA1 (Cell Signaling Technology, 2411) or TGN46 (Abcam, ab50595) were diluted with DMEM10 (1:250) and incubated with the coverslips overnight at 4°C.Secondary antibodies were diluted with TBS containing 0.2% Tween-20 and 3% BSA or DMEM10 and incubated with coverslips for one hour at room temperature.

    Techniques: Solvent